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Fig. 5
Fgf20a directs formation of the distal blastema. (A,B) ISH analysis of the distal blastema marker msxc in fgf20a-overexpressing Tg and wild-type (WT) fins at 4dpa. The msxc expression in the fgf20a-overexpressing Tg fins was broader than that in WT fins (n=10/10). (C,D) ISH analysis of junbl, an earlier blastema marker. junbl expression showed striking upregulation in the Tg fins (n=10/10). The colour reaction was terminated at the same time in the Tg and WT fins. Brackets, the approximate area of the blastema (200µm from the distal tip). Scale bars: 200µm (left); 50µm (right).
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