ZFIN Image: Trikić et al., 2011, Fig. 4

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Figure Caption

Fig. 4

Effect of knockdown.

A. Effect of morpholino-induced knockdown of cd63 on hatching. Buff = buffer/mismatch injected control. Units of time are hours. Data from two experiments each using MO2 and 4 and a combined total of 671 embryos. B. MO4 mediated band shift. 1% Agarose gel of RT-PCR products from 48 hr LWT embryos using primers corresponding to full length cd63 transcript or ef1, a ubiquitous transcription factor used as a control. The 3 lanes next to the ladder are from embryos injected with 10 pg MO4, remaining lanes are from mismatch MO3 injected embryos. Similar results were seen in the 30 MO4 injected embryos tested.

Figure Data

Phenotype details

Acknowledgments

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