ZFIN Image: Benato et al., 2014, Fig. 3

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Figure Caption

Fig. 3

DEX-induced fluorescence in Tg(9×GCRE-HSV.Ul23:EGFP)ia20 zebrafish line is dose-dependent. Top panel: EGFP protein (on the left) and mRNA (on the right) distribution after treatment of transgenics with 10, 50, 100, 250 nM and 1, 2.5, 5, 10 and 25 µM DEX for 24 h as compared to their ethanol vehicle-treated siblings. Bottom panel: Fold changes in gene expression of fkbp5 and egfp, in 10, 50, 100, 250 nM and 1, 2.5, 5, 10 and 25 µM DEX-treated transgenics compared to controls (set at 1) with vehicle only. The expression levels of the target genes were normalized on ef1a as housekeeping gene. The experiment was repeated three times with 10 embryos for each treatment. Values represent the mean ± S.E. Asterisks indicate that expression levels are significantly different from the control: **P < 0.01, ***P < 0.001. Scale bar: 200 µM.

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Expression / Labeling details

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 392(1-2), Benato, F., Colletti, E., Skobo, T., Moro, E., Colombo, L., Argenton, F., Dalla Valle, L., A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish, 60-72, Copyright (2014) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.