ZFIN Image: Wang et al., 2016, Fig. 3

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Figure Caption

Fig. 3

FCCS measurements reveal that Lefty has higher affinity to Sqt compared to Cyc.

(A) Constructs used for injection. S, signal peptide; Pro, pro-domain; Mat, mature-domain. Red arrows indicate the convertase cleavage sites. (B) Injection procedure. (C) Confocal image of an injected embryo at 30% epiboly showing the expression patterns of the fusion proteins. Scale bar represents 50 µm. (D, E, F) Representative auto- and cross-correlation functions (ACF; CCF) and fittings. (G, H, I) Individual Ln(K_d) frequency histogram and Gaussian fits (red curve). Inset, concentration plot and linear regression (red line). X axis, concentration of bound protein (C_gr(x10^-9 M)); Y axis, products of concentrations of free proteins (C_g x C_r(x10^-17 M)). n = number of data points (i.e., number of embryos).

Acknowledgments

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