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Fig. 1
Alcian blue staining suggests a correlation between sulfated proteoglycan levels and chondrocyte differentiation. Regions of cartilage that have mature chondrocytes, such as the middle of this developing zebrafish hyosymplectic (A, arrow), show decreased levels of Alcian blue staining. Compared to wild-type levels of Alcian blue staining of the developing zebrafish ceratohyal (B), proteoglycan synthesis mutants, such as this fam20b mutant, have decreased levels of Alcian blue staining and also early chondrocyte maturation and perichondral bone (C, arrow).
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