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Fig. 1
Depletion of LamC1 and Fn1 lead to severe disruption of neural tube. A-A′′: Expression of laminin (Lam) from neural plate to neural tube stages seen in transverse sections. B-B′′: Expression of Fibronectin (Fn) from neural plate to neural tube stages seen in transverse sections. C: Wild-type embryos showing normal expression of the apical marker zonula occludens (ZO-1, red) by 24 hpf. D,E: Knockdown of either Fn1 or LamC1 results in only minor disruption to ventricle morphology as revealed by ZO-1 expression. F: Knockdown of both Fn1 and LamC1 results in more severe disruption to ventricle and neural tube morphology. The normally midline expression domain of ZO1 becomes fragmented and ectopic (yellow arrowheads). Mes, mesoderm; np, neural plate; IV, fourth ventricles in the hindbrain; OV, the otic vesicle; hpf, hours post fertilization.