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Fig. 2

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ZDB-IMAGE-160511-9
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Figures for Fischer et al., 2003
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Fig. 2

ika encodes fgf24. (A) Mapping data for ika with the SSLP marker z5435. The upper gel shows the PCR products amplified from 24 siblings, while the lower gel shows the products amplified from 24 ika mutant embryos. The mutant embryos only show the (upper) Tuebingen band, except for a single embryo, which also shows the (lower) WIK band (arrow). This is the only recombination event we observed with this marker out of 938 embryos tested. (B) Schematic representation of the region of linkage group 14 to which ika and fgf24 map, depicting the genetic map on the left, and the radiation hybrid (RH) map on the right. (C) Schematic representation of the alterations to the Fgf24 protein found by sequencing fgf24 in the two ika alleles, ikahx118 and ikatm127c. (D) The phenotype generated by injecting a morpholino targeted against the translation start site of fgf24. These embryos lack pectoral fins, but otherwise appear normal, thus phenocopying ika (compare with Fig. 1A,B). Scale bar: 100µm.

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