Figures for Ahuja et al., 2016

Figure Caption/Comments:

Fig. 3

id4 mutant larvae exhibit retrograde blood flow after heat stress conditions. Embryos from an incross of id4 heterozygous animals were exposed to heat stress at 48 hpf for 1 h at 37 ºC, followed by bright field spinning disk microscopy. (A, B) Single frames (at end of ventricular systole) from blood flow movies of id4+/+ and id4-/- siblings respectively. (C) Quantification of embryos showing retrograde flow. Scale bars, 20 µm. A, Atrium; AVC, Atrioventricular Canal; V, Ventricle. Blood flow movies of id4+/+ (id4 wt video 1) and id4-/- (id4 mut video 2) are available in supplementary information.

Figure Data:
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