|ZFIN ID: ZDB-IMAGE-160427-2|
Generation of an id4 loss-of-function allele. (A) Left and right TALEN arms attached with FokI DD and FokI RR were targeted to the genomic region corresponding to the bHLH domain of Id4. (B) id4bns18, a 5 bp deletion allele, was recovered post TALEN mutagenesis. (C) This 5 bp deletion is predicted to lead to the formation of a 72-amino-acid-long truncated protein (p.Val73*). (D) Alterations in the High Resolution Melt analysis were used to determine the id4 genotype. (E, F) Overall morphology of id4-/- larvae is indistinguishable from their id4+/+ siblings at 72 hpf. RVD: repeat-variable diresidue, N: Nucleotide.
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Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.