ZFIN Image: Koenig et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Vegf signaling is necessary for intestinal vascularization. Confocal (A-C) and brightfield images (A′-C′) of live Tg(etv2:mCherry) embryos at 52 hpf. (B) 2.5 µM SU5416 treatment from 32 to 52 hpf results in near complete loss of SIV as well as nearly complete regression of ISVs as compared to the DMSO treated control (A), while milder defects are observed in both ISVs and SIV in embryos treated from 44-52 hpf (C). Arrows indicate fully formed (A) or fragmented SIV (B and C). Treated embryos display pericardial edema but otherwise are morphologically normal (A′-C′). Anterior-left, dorsal-top. Boxed region in brightfield images demonstrates area shown by confocal imaging.

Acknowledgments

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Reprinted from Developmental Biology, 411(1), Koenig, A.L., Baltrunaite, K., Bower, N.I., Rossi, A., Stainier, D.Y., Hogan, B.M., Sumanas, S., Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein, 115-27, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.