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Fig. 3
Forced expression of Hif-3α2 causes LR asymmetry defects..Embryos injected with GFP or Hif-3α2 capped mRNA (600 pg) were raised and analyzed by in situ hybridization. (A,B) Somite development was visualized by myod1 (A) and myog (B) mRNA expression at 12-14 hpf. The embryos were scored based on the criteria shown in the left panel. The quantification results are shown in the right. The total embryo number is shown on the top of each column. (C) Altered expression of spaw (C) mRNA at 18 hpf and lft2 mRNA (D) at 20 hpf. (E) The cardiac tube looping was visualized by cmlc2 mRNA expression at 48 hpf. Representative views are shown in the left panel. The quantification results are shown in the right panel. Hif-3α1 (stabilized Hif-3α1, 800 pg) injected embryos were used as controls. (F) Changes in liver and pancreas location. Hif-3α2 capped mRNA was injected into LiPan transgenic embryos. Liver (red) and pancreas (green) location was examined at 96 hpf.