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Fig. S1 Expression patterns of EGFP mRNA in the tbx16 MO- or pea3 MO-injected embryos. (A-C). Whole-mount in situ hybridization at 60% epiboly. MO specific to tbx16 was injected into the fertilized embryos of Tg(hes6:EGFP-SV40) at a concentration of 1.0 µg/µl. MO specific to pea3 at a concentration of 2.0 µg/µl. Lateral views. The percentage of embryos showing the expression patterns of EGFP shown in the panels relative to those of injected EGFP-positive embryos (n) is shown at the bottom-right of each panel. All images were taken at the same magnification. Scale bar, 200 µm.
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Reprinted from Developmental Biology, 409(2), Kawamura, A., Ovara, H., Ooka, Y., Kinoshita, H., Hoshikawa, M., Nakajo, K., Yokota, D., Fujino, Y., Higashijima, S.I., Takada, S., Yamasu, K., Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR, 543-54, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.