ZFIN Image: Kawamura et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Enhancer activities of the PT enhancer were regulated by T-box and Ets transcription factors in zebrafish embryos. (A, B) tbx16 mRNA at a concentration of 200 ng/µl was injected into fertilized embryos produced by crossing Tg(hes6:EGFP-SV40pA) and wild type fish. EGFP expression was determined by whole-mount in situ hybridization at 60% epiboly. (C, D) Injection of tbx16-EnR mRNA at a concentration of 100 ng/µl in Tg(hes6:EGFP-SV40pA) embryos. EGFP expression was determined by whole-mount in situ hybridization at 60% epiboly. (E, F) Injection of wild type pea3 mRNA at a concentration of 100 ng/µl in the Tg(hes6:EGFP-SV40pA) embryos. (G, H) Injection of pea3-EnR mRNA at a concentration of 100 ng/µl reduced expression of EGFP mRNA. EGFP expression was examined by whole-mount in situ hybridization at 60% epiboly. In all panels, lateral view. The percentage of embryos showing the expression patterns of EGFP shown in the panels relative to those of injected EGFP-positive embryos (n) is shown at the bottom-right of each panel. All images were taken at the same magnification. Scale bar, 200 µm.

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Reprinted from Developmental Biology, 409(2), Kawamura, A., Ovara, H., Ooka, Y., Kinoshita, H., Hoshikawa, M., Nakajo, K., Yokota, D., Fujino, Y., Higashijima, S.I., Takada, S., Yamasu, K., Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR, 543-54, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.