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Fig. S5
Inhibition of BMP signaling interferes with loss of cardiomyocyte differentiation markers and re-expression of a fetal form of myosin heavy chain. Related to Figure 6.
(A) Inhibition of BMP signaling either by treatment with 5 µM Dorsomorphin in myl7:GFP transgenic hearts or by noggin3 overexpression for 4 days in myl7:GFP; hsp70l:nog3 double transgenic hearts does not alter wound size (the ventricular area lacking GFP expression) at 7 dpi. Error bars represent SEM. Student’s t test, p = 0.49 (wild-type vs hsp70l:nog3), p = 0.23 (DMSO vs Dorsomorphin).
(B) Treatment of myl7:GFP transgenic fish with 5 µM Dorsomorphin for 4 days reduces the area of ventricular myosin heavy chain (Vmhc) expression in cardiomyocytes at 7 dpi. In processed Vmhc LUT images, all Vmhc signal with pixel intensity lower than 65 (8-bit image) were considered background. Error bars represent SEM. Scale bar, 50 µm. Student’s t test, p = 0.006.
(C) Immunofluorescence analysis reveals wound size at 21 dpi on heart sections of hsp70l:nog3; myl7:GFP (GFP+) and hsp70l:bmp2b (positive for myosin heavy chain staining) transgenics and their respective wild-type siblings subjected to the depicted heat-shock regime. Size of the wound tissue relative to the whole ventricle normalized to wild-type siblings is plotted. Error bars represent SEM. Student’s t test, p = 0.25 (nog3), p = 0.14 (bmp2b).
Reprinted from Developmental Cell, 36(1), Wu, C.C., Kruse, F., Vasudevarao, M.D., Junker, J.P., Zebrowski, D.C., Fischer, K., Noël, E.S., Grün, D., Berezikov, E., Engel, F.B., van Oudenaarden, A., Weidinger, G., Bakkers, J., Spatially Resolved Genome-wide Transcriptional Profiling Identifies BMP Signaling as Essential Regulator of Zebrafish Cardiomyocyte Regeneration, 36-49, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell