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Fig. 5

ID
ZDB-IMAGE-160226-17
Source
Figures for Wu et al., 2016
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Figure Caption

Fig. 5

BMP Signaling Is Required for Myocardial Regeneration and Is Sufficient to Enhance It

(A) Expression of noggin3 in hsp70l:nog3 transgenics for 6 hr is sufficient to significantly reduce nuclear pSmad1/5/8 accumulation in cardiomyocytes (CMs; positive for myosin heavy chain, MHC) at the wound border at 7 dpi. h.s., heat shock. Average percentage of cardiomyocytes expressing pSmad1/5/8 relative to that in wild-type hearts is plotted. Error bars represent SEM. Student’s t test, **p = 0.003. Scale bar, 25 µm.

(B) GFP immunofluorescence analysis reveals wound size (dashed lines) at 21 dpi on heart sections of hsp70l:nog3; myl7:GFP, and hsp70l:bmp2b; myl7:GFP double transgenics and their respective myl7:GFP-only siblings (labelled “wild-type”) subjected to the depicted heat-shock regime. Size of the GFP-negative area relative to the whole ventricle and normalized to heat-shocked myl7:GFP-only siblings is plotted. Error bars represent SEM. Student’s t test, **p = 0.0081 (nog3), *p = 0.018 (bmp2b). Scale bar, 100 µm.

(C) Acid fuchsin orange G (AFOG) staining reveals wound size (dashed lines) at 21 dpi on heart sections of hsp70l:nog3, hsp70l:bmp2b transgenics and wild-type siblings subjected to the depicted heat-shock regime. Size of the wound tissue (red and purple staining) relative to the whole ventricle and normalized to heat-shocked wild-type siblings is plotted on the right. Error bars represent SEM. Student’s t test, *p = 0.002 (nog3), *p = 0.016 (bmp2b). Scale bar, 100 µm.

Acknowledgments
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Reprinted from Developmental Cell, 36(1), Wu, C.C., Kruse, F., Vasudevarao, M.D., Junker, J.P., Zebrowski, D.C., Fischer, K., Noël, E.S., Grün, D., Berezikov, E., Engel, F.B., van Oudenaarden, A., Weidinger, G., Bakkers, J., Spatially Resolved Genome-wide Transcriptional Profiling Identifies BMP Signaling as Essential Regulator of Zebrafish Cardiomyocyte Regeneration, 36-49, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell