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Fig. S4
Activation of Tg(hs:miR-101a-sp) and Tg(hs:miR-101a-pre) did not induce ectopicprogrammed cell death. Control, Tg(hs:miR-101a-sp) and Tg(hs:miR-101a-pre) hearts were injured, heat-treated and extracted at 3 dpa for analysis of programmed cell death by TUNEL staining. We observed no differences in TUNEL+ cells between control and transgenic animals. An uninjured wildtype heart treated with DNaseI served as a positive control.
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