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Fig. 3 S1
Analysis of ARF expression in zebrafish cells. (A) Immunofluorescence for Mdm2 and ARF (top) and Tp53 (bottom) in zebrafish cells (ZKS) transfected with pcDNA-ARF. ARF and Mdm2 co-localize in the nucleolus (arrow) when ARF is expressed; in cells without ARF, Mdm2 has a diffuse nuclear staining pattern (arrow head; top). Tp53 upregulation depends on ARF expression (bottom). Scale bars: 10 µm. (B) Quantification of Tp53 upregulation in zebrafish cells (ZKS) transfected with pcDNA-ARF (N = 100, p<0.01). Results are shown as mean ± standard deviation.
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