|
Fig. S6
Telomere length dynamics of cardiac cells and location of proliferating WT cardiomyocytes in the non-injured and cryoinjured heart.
(A) The top row shows representative sections of zebrafish hearts immunostained for the cardiomyocyte marker MHC (red) and the proliferation marker PCNA (green), with nuclear counterstaining with DAPI (blue). Asterisks mark the injured area. The bottom row shows the locations of proliferating cardiomyocytes. The panels depict proliferating cardiomyocytes with relatively short telomeres (<600 a.u.) in light blue and those with relatively long telomeres (> 600 a.u.) in yellow.
(B) Representative proliferating cardiomyocytes located close to the injury site (A′) and in the remote region (A′′). Arrowheads indicate PCNA-positive cardiomyocytes with short (blue arrowhead) or long (yellow arrowhead) telomeres.
(C) Telomere length dynamics in the epicardium region of uninjured and 3 dpi WT zebrafish hearts. White arrowheads indicate epicardial cells.
(D) Immunostaining of the endocardium (endothelial cell marker erg1; green) combined with Q-FISH detection of telomere length (red) in uninjured, 3 dpi and 7 dpi WT zebrafish hearts. Nuclei are counterstained with DAPI (blue).
(E) Representative histograms showing the frequency of telomere fluorescence in arbitrary units (a.u.) in endocardial cells upon cryoinjury. a.u. arbitrary units; dpi, days postinjury; hpi; hours postinjury; PCNA, proliferating nuclear cell antigen; WT, wildtype. Scale bars: 100 µm for whole heart sections and 10 µm for zooms.
Related to Figure 4 and 5.