Fig. 2

Fig. 2

Ntla and Tbx16 directly regulate dlc expression (A)–(H) at 65% epiboly ntla^-/- and spt^-/- mutants have reduced dorsal-lateral expression of dlc, which is more pronounced in ntla^-/-; spt^-/- double mutants (asterisks). The vegetal-animal width of the expression is also reduced in mutant embryos. (I)–(L) At bud stage, dlc expression in ntla^-/- mutants is similar to wild-type embryos, although somite shape is sometimes disrupted. Expression is much reduced in spt^-/- mutants and absent in ntla^-/-; spt^-/- double mutants. (M)–(P) At 12 somite stage, dlc expression is absent from the posterior in ntla^-/- mutants, but remains in the somites. In spt^-/- mutants, expanded expression is seen in the tailbud, while expression is absent in ntla^-/-; spt^-/- double mutants. (Q)–(T) Normal expression of dlc is seen in embryos injected with Ntla-GR or Tbx16-GR and treated with cycloheximide (CHD). Addition of dexamethasone (DEX) together with cycloheximide, results in expanded dlc expression at the margin, indicating dlc is directly regulated by Ntla and Tbx16. (A)–(D) show animal views, dorsal to the left; (E) - ((H) and (Q) - (T) show dorsal views, animal to the top. (I) - (P) show dorsal views, anterior to the top.