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Fig. 1
Tricaine and isoeugenol co-operate towards healthier immobilization.
(A) Heat map of percent immobile for 48 combinations of tricaine (0–200 µg/ml) and isoeugenol (0–0.003% v/v). Embryos were dechorionated and soaked from 24–27 hpf when they were assayed for immobility. (B) Continuation of treatments from (A), embryos were assayed for immobility at 72 hpf. (C,D) Representative micrographs of control (C) and 200 µg/ml tricaine treated (D) embryos. Arrow in (D) shows failure of semicircular canal projection fusion. Asterisk in (D) shows pericardial edema. (E) Heat map of percent of embryos with pericardial edema at 72 hpf. (F) Percent control otic vesicle diameter (OVD) was calculated by dividing the average of 10–30 experimental embryos by the average of 10–30 control embryos. Heat map of percent control OVD for the combinatorial treatments. OVD was measured at 72 hpf using micrographs like those in (C, D). Percentage is based on normalization to untreated control. (G) Merge of heatmaps from (A, 27 hpf) and (F) that highlights the tradeoffs between embryo immobility and healthy development.