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Fig. S7

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ZDB-IMAGE-151001-16
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Figures for Yang et al., 2015
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Figure Caption

Fig. S7

plk2b morphants develop normally without apparent developmental delays. (A-C′′) Fluorescence and bright-field micrographs show that injection of (B–B′′′) plk2b MO1 and (C–C′′′) plk2b MO2 in Tg(kdrl:mcherry-ras) zebrafish embryos results in underdeveloped intersomitic vessel sprouting (open arrowheads), without general morphological abnormalities in whole embryo development at 48 hpf. (A–A′) control MO (n=76/76); (B–B′) plk2b ATG MO (MO1) (n=51/83); (C–C′) plk2b splice MO (MO2) (n=35/87). Scale bar, 500 µm. A′′–A′′′, B′′-B′′′, C′′–C′′′ panels are enlarged images of the boxed area of A–C, A′–C′, respectively. Scale bar, 200 µm. Top, dorsal longitudinal anastomotic vessel (yellow asterisk); bottom, dorsal aorta/cardinal vein.

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Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.