ZFIN Image: Iyengar et al., 2015, Fig. S1

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Figure Caption

Fig. S1

miniCoopR based ablation

Schematic of the miniCoopR vector (left). Promoters of interest were cloned into miniCoopR to drive expression of NTR or other genes. A recombined miniCoopR clone was injected into mitfa(If) single-cell zygotes, and embryos with rescued melanocytes were selected at three days post-fertilization; scale bar = 250 µM. These embryos were grown, and animals with melanocyte rescue were selected again at two months of age; scale bar = 5 mm.

(B) Table describing promoters tested.

(C) Adult zebrafish exressing Tg(miniCoopR-mitfa:NTR) in melanocytes and cells lineally related to them (top). Strip melanocytes were ablated when treated with neocuproine for one day (middle). After neocuproine was washed out stripe melanocytes regenerated (bottom), indicating that animals with miniCoopR-rescued melanocytes were capable of regeneration. Scale bar = 5 mm. n = 3 fish; representative images are shown. Related to Figure 1.

Acknowledgments

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Reprinted from Developmental Cell, 33(6), Iyengar, S., Kasheta, M., Ceol, C.J., Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells, 631-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell