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Fig. 2
Unpigmented mitfa-Expressing Cells Are Present at the Onset of Regeneration
(A) Flank of adult zebrafish expressing miniCoopR-mitfa:nlsEGFP. Unpigmented nlsEGFP-positive nuclei (yellow arrowheads) were admixed with nuclei of differentiated melanocytes (white arrowheads). mC, miniCoopR; BF, brightfield. Scale bar, 100 µM.
(B) Flank of adult zebrafish coexpressing mitfa:nlsEGFP and miniCoopR-mitfa:mCherryCAAX. Unpigmented cells with nlsEGFP-positive nuclei (yellow arrowheads) were distinct from differentiated melanocytes (white arrowheads). mC, miniCoopR. Scale bar, 100 µM.
(C) Flank of adult zebrafish expressing miniCoopR-mitfa:nlsEGFP before (left) and after (right) neocuproine treatment. Unpigmented cells with nlsEGFP-positive nuclei (yellow arrowheads) persisted whereas differentiated melanocytes (white arrowheads) died after neocuproine treatment. n = 5 fish; representative images are shown. mC, miniCoopR; BF, brightfield; neo, neocuproine. Scale bar, 100 µM.
(D) Flank of adult p53(lf) zebrafish coexpressing miniCoopR-mitfa:NTR and mitfa:nlsEGFP before (left) and after (right) Mtz treatment. Unpigmented cells with nlsEGFP-positive nuclei (yellow arrowheads) were spared while differentiated melanocytes (white arrowheads) were ablated following Mtz treatment. n = 3 fish; representative images are shown. Fish were treated with epinephrine prior to imaging. mC, miniCoopR; BF, brightfield. Scale bar, 100 µM.
See also Figure S2.
Reprinted from Developmental Cell, 33(6), Iyengar, S., Kasheta, M., Ceol, C.J., Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells, 631-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell