ZFIN ID: ZDB-IMAGE-150528-15
Figures for Lenard et al., 2015

Figure Caption/Comments:

Fig. S1

SIV outgrowth involves single cell assembly and angiogenic sprout fusion.

Stills of time-lapse movies showing outgrowth of the SIVs between ~36 and ~60 hpf in transgenic embryos: Tg(fli1a:EGFP) in A and Tg(fliep:GFF)ubs3,(UAS:mRFP), (5xUAS:cdh5-EGFP)ubs12 in B and C. (A) SIV emerges from the PCV at ~36 hpf (1–2). Single cells sprout ventrally from the PCV (2–3, arrows). The cells that contact each other (4, arrows) often lose contact with the PCV. A single, nonlumenized primary SIV forms and produces ventral angiogenic sprouts (5, arrows) and eventually lumenizes (6). PCV, posterior cardinal vein; DA, dorsal aorta; CCV, common cardinal vein; ISV, intersegmental vessel; DLAV, dorsal longitudinal anastomotic vessel. See also S3 Movie. (B) Multiple angiogenic sprouts originating from the primary SIV connect to each other (arrows) before (1–3) and after (4–6) lumen inflation, forming a net of vascular loops. (C) Close-up of SIV sprout fusion including the VE-cad-EGFP channel, showing new contact formation (2, arrow marks a spot of junctions), contact expansion (3, a ring of junctions), and transformation of the new branch into a multicellular tube (lines of junctions in 4). See also S4 Movie. Scale bars: 50 µm (A and B) and 10 µm (C).

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.