ZFIN Image: Lepage et al., 2014, Fig. 8

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Figure Caption

Fig. 8

Dynamin maintains EVL integrity and epiboly through the regulation of Rho A. ((A)(D)) Live images of epiboly in control injected embryos ((A)(C)) and embryos expressing DN-Rho (D) when left untreated (A), treated with DMSO (B) or treated with dynasore ((C) and (D)). ((E)(L)) Phalloidin staining of embryos injected and treated as in ((A)(D)). High magnification images in ((I)(L)) are taken from the animal pole and nuclei are marked by H2A mCherry. Arrowhead in (K) indicates a constricted cell that has an intact nucleus. (M) Quantification of the rescue of dynasore-induced epiboly delay by expression of DN-Rho. Bars represent the percentage of embryos displaying an epiboly delay±standard deviation. Statistical significance was determined by t-test, P<0.0001.

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Reprinted from Developmental Biology, 385(1), Lepage, S.E., Tada, M., and Bruce, A.E., Zebrafish Dynamin is required for maintenance of enveloping layer integrity and the progression of epiboly, 52-66, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.