ZFIN Image: Nakayama et al., 2013, Fig. S9

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Figure Caption

Fig. S9

Overexpression of gbx2 and en-gbx2 elicited dorsalization of embryos. Embryos were injected with mRNA for egfp (100 pg/embryo), gbx2 (30 pg/embryo), en-gbx2 (30 pg/embryo), or vp-gbx2 (100 pg/embryo). Lateral views with dorsal to the right and anterior to the top. (a–d) Bud-stage embryos with percentages showing extended morphology. (e–l) Expression of dorsoventral marker genes in 60%-epiboly embryos injected with gbx2-related genes. Ventral expression of eve1 was reduced by gbx2 and en-gbx2, whereas expanded by vp-gbx2. Dorsal expression of chd showed opposite responses to the same genes. Percentages show the embryos with the phenotypes shown in respective panels. Scale bars, 200 µm.

Acknowledgments

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Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.