ZFIN Image: Nakayama et al., 2013, Fig. S5

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Figure Caption

Fig. S5

Transient nature of hsp70l:gbx2 induction. (A) Offspring embryos from a cross between wild-type and hsp70l:gbx2^+/- Tg fish were exposed to heat shock at the bud stage and examined 0 h, 1 h, or 2 h later for gbx2 expression by whole mount in situ hybridization (hours post-heat shock, hph). The expression of endogenous gbx2 is marked with solid triangles. Both the intensity of staining and proportion of stained embryos were significantly reduced in 2 h. Scale bars, 200 µm. (B) Quantitative analysis of the gbx2 mRNA level by qPCR after hsp70l:gbx2 induction. The quantities of gbx2 mRNA are shown relative to those of wild-type embryos that were treated likewise. The levels of endogenous gbx2 mRNA, which was quantitated by amplification of the 3′UTR region, were less than 2% of the total gbx2 mRNA whether or not treated with heat shock (data not shown).

Acknowledgments

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Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.