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Fig. S4
Inducibility of gbx2 during the development of Tg(hsp70l:gbx2) embryos. Offspring embryos from a cross between wild-type and hsp70l:gbx2+/- Tg fish were exposed to heat shock at the specified stages and immediately examined for gbx2 expression. (A) Whole mount in situ hybridization revealed that gbx2 was always ectopically induced in approximately half of treated embryos throughout the body. (Upper three rows) Dorsal views with anterior to the top. (Bottom row) Lateral views with anterior to the left and dorsal to the top. Scale bars, 200 µm. (B and C) Quantitative analysis of the gbx2 induction. gbx2 mRNA was quantitated by qPCR in heat-treated embryos that were shown by genotyping to possess hsp70l:gbx2 or not (20 individuals each). The quantities of mRNA (coding region and 3′-UTR)(B and C, respectively) are shown relative to that in wild-type bud-stage embryos that were also subjected to HS. (B) The data show that gbx2 is always induced significantly from the shield stage to 18-somite stage. (C) The level of endogenous gbx2 mRNA, which was quantitated by amplification of the 3′-UTR region, was only marginally upregulated by HS.
Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.