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Fig. 2
Effects of overexpression of gbx2 and gbx2-ΔNCR on regionalization of the neural plate at the end of epiboly. Embryos were injected with gbx2 mRNA of 5 pg/embryo (low dose) or 30 pg/embryo (high dose) and gbx2-ΔNCR mRNA of 100 pg/embryo mRNA and examined at the bud stage for the expression of brain regional marker genes shown on the left. The most striking phenotypes and their proportions are shown in the respective panels. The positions of the MHB are marked with small triangles. Scale bars, 200 µm.
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Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.