ZFIN Image: Nakayama et al., 2013, Fig. 11

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Figure Caption

Fig. 11

Effects of modified gbx2 genes on brain regionalization in the neural plate. (A) Embryos were injected with mRNA for egfp (100 pg/embryo), gbx2 (30 pg/embryo), en-gbx2 (30 pg/embryo), or vp-gbx2 (100 pg/embryo) and examined for the expression of brain regionalization genes at the bud stage. The proportions of the defects and total numbers of scored embryos are shown in the respective panels. The positions of the MHB are indicated by small triangles. Ectopic or expanded expression is marked by asterisks. Scale bar, 200 µm. (B) qPCR analysis of the effects of gbx2-related genes on brain patterning genes. The expression of brain patterning genes was examined in triplicate by qPCR analysis. Differences from control expression (egfp) were statistically tested by the Student’s t-test. p < 0.05; p < 0.01.

Acknowledgments

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Reprinted from Mechanisms of Development, 130(11-12), Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K., Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner, 532-52, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.