Fig. 5

Fig. 5

Role of myeloid cells for the survival of regenerative cells. (A) TUNEL analysis of amputated fin fold in the vlad tepes mutant that lacks the red blood cells (n=21) and sibling wild-type (n=8) at 12 hpa. No significant increase of apoptotic cells was observed. (B) TUNEL analysis of amputated fin fold in the wild-type larvae treated with VEGFR inhibitor, PTK787 (n=39) or control vehicle (n=9). Impairment of blood vessel formation by the inhibitor did not induce apoptosis. (C) TUNEL analyses of amputated fin fold in larvae injected with the pu.1 MO (n=23) and control standard MO (n=7). Note the increase of TUNEL-positive cells in the pu.1 MO-injected larvae. (D) Quantification of TUNEL analyses (brackets) in (A–C). Data are presented as mean±s.e.m. Statistical significance was tested using Welchós t test. **P<0.001. N.S., not significant. (E) BrdU analyses of amputated fin fold in larvae injected with the pu.1 MO (n=9) and control standard MO (n=10). (F) Quantification of BrdU analyses (brackets) in (E). Data are presented as mean±s.e.m. Statistical significance was tested using Welchós t test. **P<0.001. The dotted line indicates the outline of the fin fold. Scale bars represent 100 µm.