ZFIN Image: Zhang et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Histology and apoptosis analysis of the gonads of the TG3 adults.

(a) Histology of the testes and ovaries from WT, TG3-1 and TG3-2 individuals by HE staining at 4.5 months of age. The lobular cavities in gonad of TG3-1 and TG3-2 males were observed to be in a single-row, were smaller than those of the WT males and contained none or very few mature sperm. When compare to the WT females, most oocytes were at stage I and widely dispersed throughout the gonads of the TG3-1 female fish, and at stage III and loosely arrayed in the gonads of the TG3-2 females. (b) Results of TUNEL staining of the testes and ovaries from the WT, TG3-1 and TG3-2 individuals. Multiple clusters of apoptotic spermatocytes were present in the testes of TG3-1 and TG3-2 males and some apoptotic oocytes were also present in the ovaries of TG3-1 and TG3-2 females. The red dotted line and black arrows indicate germ cells that were undergoing apoptosis. (Scale bars: 100µm).

Acknowledgments

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