ZFIN Image: Zhang et al., 2015, Fig. 3

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Figure Caption

Fig. 3

Validation of the migration, number and apoptosis of PGCs in the TG5 and the WT embryos.

(a) Schematic of the expression gene cassette of the plasmids used to establish the TG4 line. (b) A part of PGCs labeled by GFP (red arrows) migrated abnormally or lagged behind in the TG5 embryos at 10 hpf and 13 hpf, compared with the WT embryos. (c) The number of PGCs decreased in the TG5 embryos at 20 hpf and 24 hpf compared with the WT embryos. The red arrow indicates the location of PGCs. (d) WT embryos contained approximately four times the number of PGCs as the TG5 embryos (detected by GFP fluorescence intensity of PGCs at 24 hpf (n = 10). (e) Apoptosis of some PGCs was detected (red arrows) within some of the TG5 embryos. (Scale bars: 100µm).

Acknowledgments

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