IMAGE

Fig. S4

ID
ZDB-IMAGE-141124-12
Source
Figures for Schindler et al., 2014
Image
Figure Caption

Fig. S4

Normal islet1 expression at the venous pole in embryos overexpressing hand2. (A-B) Immunofluorescence at 36 hpf for MF20 (red, visible throughout the heart) and Islet1 (green, visible in the nuclei of a subset of atrial cells). Frontal views, dorsal to the top; arrows point to the Islet1-positive population at the venous pole of the atrium. Following heat shock at 10 hpf, the population of Islet1-positive cells at the venous pole appears similar in hearts from (A) nontransgenic embryos and (B) Tg(hsp70:hand2) embryos. (C) Bar graph compares average number of Islet1-positive cardiomyocytes at 36 hpf in nontransgenic embryos and Tg(hsp70:hand2)embryos, following heat shock at 10 hpf. Error bars indicate standard deviation; no significant difference is observed between these two data sets (n=12, p=0.09). (D-E) In situ hybridization depicts islet1 expression at 36 hpf, following heat shock at 10 hpf. Frontal views, dorsal to the top; arrows point to the ring of islet1-expressing cells at the venous pole of the atrium. Expression patterns are similar in (D) nontransgenic embryos and (E) Tg(hsp70:hand2) embryos.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development