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Fig. S12
Impact of UV irradiation on embryonic SA-β-gal activity in p53 and/or spns1 mutants. (A) Effect of UV treatment on embryonic SA-β-gal activity was validated in spns1-mutant animals with normal or mutant p53 backgrounds. The UV (18 mj/cm2) treatment was done at 68 hpf, followed by the phenotype observations at 72 hpf. Scale bar, 250 μm. (B) Quantification of the SA-β-gal intensities shown in (A). Quantification of data presented in panel A (n = 12) is shown in the right graph; the number (n) of animals is for each genotype. Error bars represent the mean ± S.D., **p<0.05; *p<0.01; ns, not significant.
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