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Fig. S7
Suf/Spastizin is essential for vesicle fission. (A) Accumulation of Clathrin-coated cisternae in suf/spastizin mutants. Electron micrographs showing cellular compartments from wt (top row) and suf/spastizin mutants (lower row). The cortex region is marked by the fenestrated area in some panels, which represents the zona radiata forming the chorion membrane after fertilization. Bottom panels show vesicular compartments accumulating Clathrin buds on their membrane in suf/spastizin mutants. Picture frame: 1 μm (left column) 2 μm (middle and right column). (B–D) Dynamin and Clathrin accumulation on cortical granules of suf/spastizin mutants. (B) Confocal sections comparing Clathrin localization (green) in wt and suf/spastizin mutants. (C) Confocal sections comparing Dynamin localization (green) in wt and suf/spastizin mutant oocytes. Left column shows optical section in the oocyte center. Middle column: optical section of cortex region. Right panel: Cortical section showing the accumulation of Dynamin (green) in mutants. (D) Colocalization of Clathrin (green) or Dynamin (green) on cortical granules (red). Left panel of Clathrin staining shows the cortex region and right panel shows the center of the oocyte. Dynamin panel shows cortex region. Scale bar: 50 μm. Small icon next to figures indicates the level of the optical section (black line) in the oocyte (blue circle).