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Fig. 3
Analysis of Fgf8 internalization Clathrin coated vesicles and early endosomes in fish fibroblasts.
Confocal analysis of Fgf8-GFP co-localization with indicated early endocytic markers in zebrafish PAC2 cells. Columns 1-3 show confocal images and columns 4-5 shows bright field images merged with the confocal image. Co-localization of Fgf8 with Clathrin (A-D′) and with Rab5 (E-H′) was investigated. Furthermore, insets show the increasing size of Rab5 positive early endosomes upon Hsc70 overexpression (E and G).
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