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Fig. 3
Reproducible Venus expression patterns upon targeted integration of EL161 enhancer construct demonstrate cis-regulatory function in the brain. (A) Brain-specific enhancer effect of a transgene inserted in different integration sites. Domains of Venus activity are specified in B. Dorsal (top) and lateral (bottom) views of the head of 3 dpf F1 transgenic larvae. Scale bar: 100 μm. (B) Domains of Venus reporter expression and their distribution among targeted (PhiC31 integrase) and randomly (Tol2 transposase) transgenic lines. IF, integrase-injected founders; IP, integrase-mediated expression patterns; TF, Tol2-injected founders; TP, Tol2-mediated expression patterns. Blue depicts expression domains overlapping with esrrga activity, whereas red colour indicates additional domains. Light blue indicates weak expression.