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Fig. S7
Threshold choice and traces for regressor maps (related to Figure 7)
(A) The N-fold enrichment of the maximum absolute regression coefficient (across all regressors) for every voxel in the real data compared to the equivalent computation performed using shuffled versions of the same regressors (8 second blocks; n=1,568,168 cube ROIs from 7 fish). The value of 0.3 is more than 200 times more common in the real data, indicating that this is a conservative threshold for selecting ROIs with activity correlated to the behavior.
(B) Z-projection of voxels with activity correlated to at least one regressor at three different thresholds: 0.25 in blue, 0.275 in green and 0.3 in red. The pattern of white areas with blue borders shows that reducing the threshold changes the boundaries of identified regions and contributes noise, but does not result in the identification of more active areas, and indicates that the map is robust to the precise choice of threshold. Scale bar is 50 microns.
(C) Average normalized fluorescence traces for the functional structures shown in Figure 7. All traces are the mean across fish with SEM shown by gray shading (n=6 fish). A schematic of the four stimuli is shown. Gray boxes and dotted lines indicate the duration of each of the four stimuli in all plots.