ZFIN ID: ZDB-IMAGE-140530-14
Proximity labeling of protein pairs is specific. Proximity labeling of Harmonin and Cdh23. (A) In wild-type anterior macula, Harmonin and Cdh23 are in close proximity in hair cell bodies. Note the apical localization of the signal (yellow arrowheads). (B,C): DIC channel and merge of panels A and B. (D-I) Negative controls. No significant signal was obtained when Cdh23 expression was knocked down by injection of cdh23 morpholinos (D) or in ush1c mutants (G). (E,H) DIC channel. (F,I) Merge of D and E, and H and I respectively. WT: wild-type; MO: morpholino. Scale bar: 5 µm.
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