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Fig. 5 Expression of Shp2-D61G- and Shp2-A462T-induced L/R asymmetry defects. Embryos were injected at the one-cell stage with mRNA encoding WT-Shp2 (Shp2), Shp2-D61G or Shp2-A462T and fixed at the stage indicated. In situ hybridization was carried out using probes specific for spaw (A-D, southpaw), lft2 (E-G, lefty2) and fabp (H-K, fatty acid binding protein, marking the liver). Representative pictures are shown of Shp2-D61G-expressing embryos. lft2 expression was observed only on the left side or on the right side. Organ asymmetry as assessed using the different markers was scored for embryos injected with WT-Shp2, Shp2-D61G and Shp2-A462T. Percentages of left, middle/bilateral and right expression of the markers are depicted. The number of embryos (n) is indicated.