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Fig. 2

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ZDB-IMAGE-140421-2
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Figures for Wilkins et al., 2014
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Fig. 2

krt18-driven reporters allow live detection of the developing biliary system. (A) Fluorescent micrograph of live 6 dpf Tg(krt18:egfp) larva, showing EGFP expression in intrahepatic biliary cells (single arrow) and gallbladder epithelium (double arrow), partly obscured by skin expression. (B) and (C) Fluorescent micrographs of live 4 dpf (B) and 7 dpf (C) Tg(krt18:TRAP) larvae, showing visualization of EGFP-Rpl10a fusion protein in developing intrahepatic biliary cells (single arrows) and gallbladder epithelium (double arrow). Red dashed line in (B) indicates gallbladder overlying the liver, out of the focal plane.

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Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns