IMAGE

Fig. 2

ID
ZDB-IMAGE-140327-3
Genes
Source
Figures for Lee et al., 2003
Image
Figure Caption

Fig. 2 The no soul gene encodes a winged helix domain-containing protein that belongs to the foxi1 subfamily. (A) ABI automated sequencer-produced chromatographs showing that the Ser194 is mutated to Pro in the no soul mutant. (B) Comparative model showing that the predicted conformations of the native and mutated segment are different. The ab initio modeled segment is in green (the rest of the protein is shown in brown), the proline mutation is shown in the ball-and-stick style (red), and the bound DNA is blue. In the mutant model, the C terminus of the recognition helix is shortened by one turn and the loop connecting the recognition helix to the subsequent b strand is shifted significantly relative to the native model. These conformational changes decrease the extent of interactions between the protein and DNA, assuming DNA is positioned as in the template structures. (C) Sequence alignment between no soul/foxi1, Xenopus (xfoxi1c), mouse (Mfkh10) and the winged helix domain of genesis that was used for comparative structure modeling. The winged helix motif and the position of the no soul mutation are marked. (D) Confocal image showing the control and the foxi1 morpholino-injected embryos. Although motor neurons are normal, epibranchial placode-derived visceral sensory neurons are defective as in the no soul mutant. mV/mVII/mX, the Vth, VIIth and Xth motor neurons; OT, ocular and trochlear motor nuclei; VII, geniculate ganglion; IX, petrosal ganglion; X, nodose ganglion.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development