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Fig. 5 Dorsoventral axial defects are caused by higher-dose Nkx2.5 RNA injection. (A) Control embryo after 1 day of development. (B) A severely affected zebrafish embryo after NKx2.5 injection. The head and eyes are missing, notochord is diminished and the somites are fused. (C-F) Effects on the dorsoventral axis are evident during gastrulation, with (C,D) reduction of the expression of the dorsal marker, goosecoid, and (E,F) expansion of the domain of a ventral lateral marker, gata2 . C and E show embryos injected with 250 pg lacZ mRNA, probed with gsc and gata2 respectively. D and F show embryos injected with 250 pg zebrafish Nkx2.5 RNA, and probed with gsc and gata2, respectively.