|
Fig. S2 Cvl2 is required for neural crest specification. (A) ISH analysis of embryos injected with 4 ng control MO or cvl2 MO1 using probes against the neural crest marker snai1b. Embryos were fixed at the five-somite stage Dorsal anterior view, anterior to the top. (B) ISH on Tg(BRE:mRFP) embryos injected with 4 ng control MO or 4 ng cvl2 MO2 or MO3. Dorsal anterior views, with anterior to the top. Embryos were fixed at the three-somite stage. (C and D) Alcian blue cartilage staining of 5-day old zebrafish larvae, injected with 2 ng p53 morpholino (Robu et al., 2007) and either 4 ng control or 4 ng cvl2 MO1 (C) or 4 ng control MO or cvl2 MO2 or MO3 (D). Note that the phenotype of the cvl2 MO1 morphants may be compounded by some additional deleterious effects that manifest themselves at this late stage. The numbers indicate embryos with the observed phenotype for a representative experiment.