ZFIN Image: Fernandes et al., 2013, Fig. S3

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Figure Caption

Fig. S3 High-resolution imaging of neuroendocrine cells for cell counting. Example of 3 dpf wildtype embryos imaged at single-cell resolution for quantification of cell numbers. (A) oxt, (B), avp, (C) trh, (D) crh expression analysis by WISH. For this figure, from the whole image stack with images at 1 μm spacing, sub-stacks of planes were used to generate a series of dorso-ventral Z-projections containing the region of interest. A higher magnification of regions of interest is shown on the right panel. Dorsal view, anterior at left. Scale bar is 100 μm. H, hypothalamus; HB, Hindbrain; PO, preoptic region; PT, posterior tuberculum.

Acknowledgments

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