IMAGE

Fig. S7

ID
ZDB-IMAGE-131105-31
Source
Figures for Rosenfeld et al., 2013
Image
Figure Caption

Fig. S7

The hspb7 translation-blocker MO and hspb7 splicing-blocker MO synergize to cause jogging defects when used together at sub-threshold levels. A) Representative jogging phenotypes at 30hpf in embryos derived from the myl7:gfp reporter strain. B) Quantification of jogging phenotypes in wildtype embryos (wt, n=32), or embryos injected with the standard control MO (control, n=28), hspb7 7MO at 0.15mM (ssMO, n=81), hspb7 7MO1 at 0.075 mM (tbMO, n=66), 7MO1 + standard control (hsbp7 tbMO + control, n=82), or 7MO + 7MO1 (hspb7 tbMO + ssMO, n=78). The 7MO splice-blocker MO used at 0.15mM does not generate the left-right defects observed at the higher concentrations but does so when co-injected with a sub-threshold concentration of the translation blocker MO.

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Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.