ZFIN Image: Novikov et al., 2013, Fig. 10

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Figure Caption

Fig. 10

CP formation is repressed by forced expression of wild-type but not mutant tmem88a, and this phenotype is rescued by enhancing early WNT signaling. (A-E) WISH for nkx2.5 (box) and ntl in representative 8-somite stage uninjected control zebrafish embryos (A, Ctrl), embryos injected with wild-type tmem88a mRNA (B, 88a RNA), embryos injected with mutated tmem88a mRNA (C, Δ88a), heat-shocked uninjected control embryos (D, Ctrl+wnt8), and heat-shocked embryos that had been injected with wild-type tmem88a mRNA (E, 88a RNA+wnt8). Heat-shock treatment was administered to embryos at 3 hpf for 30 minutes. n>15 embryos analyzed per condition. (F) nkx2.5 relative expression levels assessed by qPCR. ^**P<0.01; error bars indicate s.d.

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