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Fig. 2
foxd3 mRNA rescues NCCs in prdm1a-deficient embryos. (A-F) ISH for neural crest markers snai1b (A-C) and sox10 (D-F) on 2- to 4-somite (11-12 hpf) uninjected zebrafish embryos (A,D), prdm1a morphants (B,E) and with prdm1a-MO co-injected with foxd3 mRNA (C,F). Dorsal view of WT embryos show neural crest expression at the NPB (arrows) for both snai1b and sox10, with snai1b also expressed in the adaxial cells and mesoderm. In prdm1a morphants, the expression is reduced at the NPB. However, after co-injection with foxd3 mRNA, the NPB expression is restored. All images are dorsal views, anterior to the top. a, adaxial cells; m, mesoderm. (G) Percentage of embryos expressing each marker. snai1b: WT, n (number of embryos exhibiting the phenotype in A out of the number of embryos examined)=81/84; prdm1a-MO, n=13/59; rescue, n=41/48. sox10: WT, n=156/160; prdm1a-MO, n=4/92; rescue, n=39/77. *P<0.05. Error bars indicate s.e.m.