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Fig. 2 Assay of UAS constructs in binary transgenic system. (A) Experimental scheme using the Tg(ptf1a:Gal4-VP16)jh16 driver line for transcriptional activation of 14X and 4Xnr UAS:GFP reporter transgenes. (B) Pattern of tissue-specific fluorescence in Tg(ptf1a:GFP)jh1/+ larvae at 2 dpf (Pisharath et al., 2007). (C) In the presence of the ptf1a driver, larvae from independently derived lines that carry either 14X UAS:GFP or 4Xnr UAS:GFP transgenes show a similar pattern of GFP labeling.
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Reprinted from Developmental Biology, 352(2), Akitake, C.M., Macurak, M., Halpern, M.E., and Goll, M.G., Transgenerational analysis of transcriptional silencing in zebrafish, 191-201, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.